Agricultural practitioners, researchers and policymakers are increasingly advocating for IPM to reduce pesticide use while preserving crop productivity and profitability. Using selective pesticides, putatively designed to act on pests while minimising impacts on off-target organisms, is one such option – yet evidence of whether these chemicals control pests without adversely affecting natural enemies and other beneficial species remains scarce. At present, the selection of pesticides compatible with IPM often considers a single (or a limited number of) widely distributed beneficial species, without considering undesired effects on co-occurring beneficials. To address this gap, we conducted standardised laboratory bioassays to assess the acute toxicity effects of 20 chemicals on 15 beneficial with the specific aims of identifying common and diverging patterns in acute toxicity responses of tested beneficial. Our work demonstrated that in most cases, chemical toxicities cannot be generalised across a range of beneficial insects and mites providing biological control, a finding that was found even when comparing impacts among closely related species of beetles, wasps and mites. This challenges the notion that chemical toxicities can be adequately tested on a limited number of ‘representative’ species; instead, it highlights the need for careful consideration and testing on a range of regionally and seasonally relevant beneficial species. Working closely with industry, we have utilised this dataset to develop a Beneficials Chemical Toxicity Table, specifically tailored to the Australian grains industry. First launched in 2022, this table has been well received by grain growers and advisors nationally, with annual revisions ensuring it is kept up-to-date.