Anthropogenic actions have caused irreversible damage to biodiversity and led to the current conservation crisis, the sixth mass extinction. For some species, such as the endangered pygmy bluetongue (Tiliqua adelaidensis), translocation into their historical range of Adelaide, South Australia is anticipated to mitigate against the threat of extinction. Understanding species’ interactions at different habitat scales will improve translocation success, as fauna may show a pattern of habitat use if they rely on a specific habitat component. Pygmy bluetongues rely on spider burrows (microhabitat) in grasslands (site) with some form of grazing (landscape). We aimed to assess the viability of translocating the pygmy bluetongue into its historical range of Adelaide. We used maximum entropy modelling to model future habitat suitability in Adelaide using the pygmy bluetongue, and two indicator species, brush wire grass (Aristida behriana) and the Adelaide trapdoor spider (Blakistonia aurea). We found brush wire grass was the best indicator species and identified four potential translocation sites within the Adelaide region. We compared these four potential translocation sites to four pygmy bluetongue populations at the southern, central, and northern ranges of the lizard’s current distribution for habitat assessments of known versus potential pygmy bluetongue habitat. We will discuss the results of using different indicator species during maximum entropy modelling and focus on the site level assessments, presenting insights into the inter-tussock space preferences of the lizards, invertebrate capture trials, soil structure analyses, spider burrow surveys, and their implications for future translocations of pygmy bluetongues into Adelaide.